Pakistan facing serious problem of electricity couple with handling of waste water generated from industries. In this regard study was focused to investigate the reduction of waste water from industry couple with energy generation from that source. Different blending ratio of cow dung manure was used with distillery waste water to make substrate for electricity generation in microbial fuel cell. Microbial fuel cell was used alternative environment for anode and cathode chamber. An aerobic condition for anode and aerobic condition for cathode chamber, fish pump is used for creating aerobic condition in cathode chamber for oxidation of proton coming from anode chamber through salt bridge. The maximum power production was measured at 50/50 about 2300 mv/l. and minimum 230mv/l. The addition of cow dung manure could benefit for power generation with distillery waste water.