Forensic science plays a crucial role as a component of the criminal justice system. This study aims to know about the problems police officials are facing in Forensic Criminal Investigation (FCI) in district Peshawar. Quantitative research was carried out by using 50 sample size of male Police Investigation officers through convenience sampling technique and the data was collected through interview schedule. Research findings shows that investigation officers were facing numerous problems in conducting forensic criminal investigation in district Peshawar like problems in forensic training on crime scene; untrained investigator were damaging the physical evidence; lack of Crime Scene Mobile Van. This study recommends for the provision of crime scene mobile van to the investigators, creation of forensic training schools and establishment of Forensic and DNA labswhich is important to maintain the proper low of forensic criminal investigation.