Adsorption is a well- recognized technique for treatment of wastewater streams containing petroleum effluents. The study undertaken aims to study the possibility of Neem leaves to be used as an effective biosorbents for the removal of oil from oil-water mixture. Neem leaves as biosorbents were employed to treat oily waste streams. The effects of biosorbent dosage, contact time and pH were evaluated. The optimal parameters were determined to be 2 g/L biosorbent dosage, pH 10 and equilibrium time of one hour. The adsorption of crude oil on Neem leaves powder (NLP) was best described by Langmuir isotherm and crude oil adsorption kinetics was determined to follow pseudo-second-order reaction kinetics. At optimal parameters, NLP was able to reduce crude oil concentration in tap water below the detectable limit, strongly suggesting the use of NLP as alternative to other adsorbents for oil-water emulsion treatments.