Transgender community faces insulting remarks and people treat them in a bad way. Discrimination is being faced by transgender at all levels such as at school, in family, at places where they live. The purpose of the present study was to explore the attitude of people towards third gender. A sample of 600 were selected through purposive sampling and snow ball sampling techniques. Data was collected from Two cities of Pakistan Sahiwal and Multan. Demographic variables of participants used in this research are age, gender, monthly income, qualification and marital status. Attitude towards Hijras scale was used in this research. Results showed that females have more acceptances of Hijra’s (transgender) rights and status as compared to males. Males and females don’t want any close relationship with Hijras (transgender).Sexual issues (SI) are significant in males as compared to females. Education has more impact on people’s attitude towards Hijra community. People belonging to religious groups have more negative attitude towards Hijras as compared to other groups.